THIAZOVIVIN
發(fā)布者：信康 瀏覽次數(shù)：
產(chǎn)品名稱:THIAZOVIVINCAS:1226056-71-8別名：N-芐基-2-(嘧啶-4-基氨基)噻唑-4-甲酰胺;N-芐基-2-(嘧啶-4-基氨基)噻唑-4-羧酰胺英文名：Thiazovivin
- 密度 1.379
- 儲(chǔ)存條件 Store at -20°C
- 溶解度 DMSO: soluble20mg/mL, clear
- 顏色, white to beige to brown
- 生物活性Thiazovivin是一種新型ROCK抑制劑，IC50為0.5 μM，在單細(xì)胞分離后，促進(jìn)人胚胎干細(xì)胞（hESC）的存活。
- 體外研究Although displaying little impact on cell proliferation, Thiazovivin treatment significantly enhances the survival of human embryonic stem cells (hESCs) after enzymatic dissociation more than 30-fold, while homogenously maintaining pluripotency with the characteristic colony morphology, expression of typical pluripotency markers such as alkaline phosphatase (ALP), and normal karyotype. Dissociated hESCs treated with Thiazovivin display dramatically increased adhesion to matrigel- or laminin-coated plates but not to gelatin-coated plates within a few hours. Thiazovivin treatment increases cell-ECM adhesion-mediated β1 integrin activity, which synergizes with growth factors to promote cell survival. In addition to activating integrin, Thiazovivin but not Tyrintegin (Ptn) protects hESCs from death in the absence of ECM in suspension through E-cadherin-mediated cell-cell interaction. Thiazovivin treatment potently inhibits endocytosis of E-cadherin, consequently stabilizing E-cadherin on the cell surface and leading to reestablishment of cell-cell interaction, which is essential for hESC survival in ECM-free conditions. Thiazovivin but not Tyrintegin (Ptn) at 2 μM inhibits Rho-associated kinase (ROCK) activity and protects hESCs at a similar level as the widely used selective ROCK inhibitor Y-27632 at 10 μM, suggesting that Rho-ROCK signaling regulates cell-ECM and cell-cell adhesion. Thiazovivin at 1 μM increases the reprogramming efficiency of CB mononuclear cells to induced pluripotent stem cells (iPSCs) by more than 10 times.
